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Uteroglobin/SCGB1A1 Overexpression Lysate

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Western Blot: Uteroglobin Overexpression Lysate (Adult Normal) [NBL1-15732] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Uteroglobin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Uteroglobin/SCGB1A1 Overexpression Lysate Summary

Description

Uteroglobin/SCGB1A1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202497

Accession#: NM_003357

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
SCGB1A1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
9.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Uteroglobin/SCGB1A1 Overexpression Lysate

  • Blastokinin
  • CC10
  • CC10Urine protein 1
  • CC16
  • CCPBP
  • CCSP
  • CCSPCCPBP
  • Clara cell phospholipid-binding protein
  • Clara cells 10 kDa secretory protein
  • Clara
  • PCB-BP
  • SCGB1A1
  • Secretoglobin family 1A member 1
  • secretoglobin, family 1A, member 1 (uteroglobin)
  • UGB
  • UGBUP-1
  • UP1
  • Urinary protein 1
  • Uteroglobin

Background

Uteroglobin, also known as secretoglobin family 1A member 1 (SCGB1A1), is a protein with anti-inflammatory and immunomodulatory properties. It is a member of the secretoglobin family, which includes small, secreted, disulfide-bridged dimeric proteins found only in mammals. Uteroglobin is a secretory protein induced by progesterone. It is present in the mucosal epithelia of nearly all organs and in many body fluids. The protein is also involved in anti-chemotactic, anti-allergic, anti-tumorigenic, and embryonic growth-stimulatory activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SCGB1A1