Novus Biologicals products are now on bio-techne.com

UNC13D/Munc 13-4 Antibody (1223A) [Unconjugated]

Images

 
Western blot shows lysates of NK-92 human natural killer lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of ...read more
UNC13D was detected in immersion fixed NK-92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) at 5 µg/mL for 3 hours at room temperature. Cells were ...read more
Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 1 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
1223A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

UNC13D/Munc 13-4 Antibody (1223A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
KLH-coupled N-terminal human UNC13D peptide
Accession # Q70J99
Specificity
Detects the conventional form of human UNC13D in Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
UNC13D
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB89661 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC13D/Munc 13-4 Antibody (1223A) [Unconjugated]

  • FHL3
  • HLH3
  • HPLH3
  • Munc13-4
  • Munc13-4protein unc-13 homolog D
  • unc-13 homolog D (C. elegans)
  • UNC13D

Background

UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues.  UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acids (aa) 40-1090 but have distinct N-terminal segments.  Human and mouse have 75% aa sequence identity throughout the region used as the immunogen.  It is unknown whether mice express the alternative isoform. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-86122
Species: Hu
Applications: IHC, IHC-P, WB
H00005873-M02
Species: Hu, Mu, Rb
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD, S-ELISA, WB
NBP3-17459
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-97512
Species: Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF5900
Species: Hu
Applications: WB
NBP1-84978
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-33037
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32660
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB120-19294
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00001130-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB5938
Species: Hu
Applications: ICC, WB
NBP1-84843
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
202-IL
Species: Hu
Applications: BA
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NBP1-84764
Species: Hu
Applications: IHC, IHC-P
AF8221
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
MAB89661
Species: Hu
Applications: WB, Simple Western, ICC/IF

Publications for UNC13D/Munc 13-4 Antibody (MAB89661) (0)

There are no publications for UNC13D/Munc 13-4 Antibody (MAB89661).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for UNC13D/Munc 13-4 Antibody (MAB89661) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB89661:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot UNC13D/Munc 13-4 MAB89661
Enlarge
5
reviewed by:
Verified Customer
WB Human 02/05/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman PBMC
SpeciesHuman
LotCJRJ0115091

Comments

CommentsSee attachment. Please get back to me if you require more information.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UNC13D/Munc 13-4 Antibody (MAB89661). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking Munc13-4 N terminal antibody with related epitope sequence.
    • Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
02/05/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol UNC13D
Uniprot