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Recombinant Human UBE2N/Ubc13 His Protein

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SDS-Page: Recombinant Human UBE2N/Ubc13 Protein [NBP1-51063] - 15% SDS-PAGE (3ug)
SDS-Page: Recombinant Human UBE2N/Ubc13 Protein [NBP1-51063] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human UBE2N/Ubc13 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-152 of Human UBE2N/Ubc13

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UBE2N
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human UBE2N/Ubc13 His Protein

  • bendless-like ubiquitin conjugating enzyme
  • Bendless-like ubiquitin-conjugating enzyme
  • BLU
  • EC 6.3.2.19
  • MGC131857
  • MGC8489
  • UBC13
  • UbcH13
  • UbcH-ben
  • UBE2N
  • Ubiquitin carrier protein N
  • ubiquitin-conjugating enzyme E2 N
  • ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
  • ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
  • Ubiquitin-protein ligase N
  • yeast UBC13 homolog

Background

UBE2N, also known as UBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage. Recombinant human UBE2N protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2N