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UbcH5c/UBE2D3 Antibody

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Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - MCF7, Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7.
Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.
Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.
Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.
Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.
Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Jurkat, Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.
Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Lanes: 1 : 40 ng HIS-UBE2D1 protein2: 40 ng HIS-UBE2D2 protein3: 40 ng HIS-UBE2D3 protein4: 40 ng HIS-UBE2D4 protein5: 40 ng HIS-UBE2E1 protein6: 40 ng HIS-UBE2E2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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UbcH5c/UBE2D3 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to UBE2D3(ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)) The peptide sequence was selected from the N terminal of UBE2D3. Peptide sequence MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVF. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2D3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-55276.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for UbcH5c/UBE2D3 Antibody

  • E2(17)KB 3
  • E2(17)KB3
  • EC 6.3.2.19
  • MGC43926
  • MGC5416
  • UBC4/5
  • UBC5C
  • UbcH5c
  • UBE2D3
  • Ubiquitin carrier protein D3
  • ubiquitin-conjugating enzyme E2 D3
  • Ubiquitin-conjugating enzyme E2(17)KB 3
  • Ubiquitin-conjugating enzyme E2-17 kDa 3
  • ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
  • ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UbcH5c/UBE2D3 Antibody (NBP1-55276)(1)

We have publications tested in 1 application: Immunocytochemistry/ Immunofluorescence.


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Bioinformatics

Gene Symbol UBE2D3
Uniprot