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Rat Troponin T Type 3 (fast skeletal) ELISA Kit (Colorimetric)

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ELISA: Rat Troponin T Type 3 (fast skeletal) ELISA Kit (Colorimetric) [NBP2-76603] - Samples were spiked with high concentrations of Rat Troponin T Type 3 (fast skeletal) and diluted with Reference Standard & Sample ...read more
ELISA: Rat Troponin T Type 3 (fast skeletal) ELISA Kit (Colorimetric) [NBP2-76603] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Rat Troponin T Type 3 (fast skeletal) ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat TNNT3 in samples. No significant cross-reactivity or interference between Rat TNNT3 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 4.97
Intra-Assay
%CV < 5.58
Spike Recovery
86 - 107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNNT3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Troponin T Type 3 (fast skeletal) ELISA Kit (Colorimetric)

  • Beta-TnTF
  • fast skeletal muscle
  • TnTf
  • troponin T type 3 (skeletal, fast)
  • troponin T3, skeletal, fast
  • troponin-T3, skeletal, fast

Background

Cardiac Troponin T, a 36kDa protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNNT3