I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.

Name: TrkA Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF175
Rating: 5 (1 reviews)

Question

Accepted Answer

NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Flow, IHC, B/N, CyTOF-ready
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free AF175-SP

Immunogen	Mouse myeloma cell line NS0-derived recombinant human TrkA Ala33-Glu407 Accession # AAA36770
Specificity	Detects human TrkA in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat TrkA is observed and 10% cross-reactivity with recombinant human (rh) TrkB and rhTrKC is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	NTRK1
Purity Statement	Antigen Affinity-purified
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

TrkA Antibody [Unconjugated]

Species Glossary

Images

Product Details

Order Details

TrkA Antibody [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for TrkA Antibody [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for TrkA Antibody (AF175)(15)

Review for TrkA Antibody (AF175) (1) 51

Summary

Product General Protocols

Video Protocols

FAQs for TrkA Antibody (AF175). (Showing 1 - 1 of 1 FAQs).

Secondary Antibodies

Isotype Controls

Additional TrkA Products

Blogs on TrkA.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Publications using AF175	Applications	Species
Liu S, Xiang K, Yuan F, Xiang M Generation of self-organized autonomic ganglion organoids from fibroblasts iScience 2023-03-01 [PMID: 36922996]
Viventi S, Frausin S, Howden SE et al. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia Stem cells translational medicine 3/18/2021 [PMID: 33734599]
AJ Hulme, JR McArthur, S Maksour, S Miellet, L Ooi, DJ Adams, RK Finol-Urda, M Dottori Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons Frontiers in Cellular Neuroscience, 2020-12-04;14(0):600895. 2020-12-04 [PMID: 33362470] (ICC, Human)	ICC	Human
C Castellini, S Mattioli, AD Bosco, E Cotozzolo, A Cartoni Ma, M Rende, AM Stabile, A Pistilli Nerve growth factor receptor role on rabbit sperm storage Theriogenology, 2020-05-11;153(0):54-61. 2020-05-11 [PMID: 32442740] (Flow Cytometry, Neutralization, Rabbit)	Flow Cytometry, Neutralization	Rabbit
C Castellini, S Mattioli, A Dal Bosco, G Collodel, A Pistilli, AM Stabile, L Macchioni, F Mancuso, G Luca, M Rende In vitro effect of nerve growth factor on the main traits of rabbit sperm Reprod. Biol. Endocrinol., 2019-11-12;17(1):93. 2019-11-12 [PMID: 31718673] (Flow Cytometry, ICC, Neutralization, Western Blot, Rabbit)	Flow Cytometry, ICC, Neutralization, Western Blot	Rabbit
S Desiderio, S Vermeiren, C Van Campen, S Kricha, E Malki, S Richts, EV Fletcher, T Vanwelden, BZ Schmidt, KA Henningfel, T Pieler, CG Woods, V Nagy, C Verfaillie, EJ Bellefroid Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA Cell Rep, 2019-03-26;26(13):3522-3536.e5. 2019-03-26 [PMID: 30917309] (ICC, Human)	ICC	Human
AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells Sci Rep, 2018-01-12;8(1):603. 2018-01-12 [PMID: 29330377] (ICC, Human)	ICC	Human
Wenyu Shi, Suraj Konnath George, Bhawana George, Choladda V. Curry, Albina Murzabdillaeva, Serhan Alkan et al. TrkA is a binding partner of NPM‐ALK that promotes the survival of ALK + T‐cell lymphoma Molecular Oncology 2017-09-01 [PMID: 28557340]
Sandra Pinet, Barbara Bessette, Nicolas Vedrenne, Aurélie Lacroix, Laurence Richard, Marie-Odile Jauberteau et al. TrkB-containing exosomes promote the transfer of glioblastoma aggressiveness to YKL-40-inactivated glioblastoma cells Oncotarget 2016-08-02 [PMID: 27385098]
Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L Nerve growth factor downregulates inflammatory response in human monocytes through TrkA. J Immunol, 2014-02-28;192(7):3345-54. 2014-02-28 [PMID: 24585880] (Neutralization, Human)	Neutralization	Human
Flowerdew S, Wick D, Himmelein S, Horn A, Sinicina I, Strupp M, Brandt T, Theil D, Hufner K Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection. PLoS ONE, 2013-12-19;8(12):e83603. 2013-12-19 [PMID: 24367603] (IHC, Human)	IHC	Human
Erol Saygili, Rahel Kluttig, Obaida R. Rana, Esra Saygili, Christopher Gemein, Matthias D. Zink et al. Age-related regional differences in cardiac nerve growth factor expression Age (Dordr) 2012-06-01 [PMID: 21559866]
Huang YT, Lai PC, Wu CC TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells. Int. J. Oncol., 2010-10-01;37(4):943-9. 2010-10-01 [PMID: 20811716] (Functional Assay, Human)	Functional Assay	Human
Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells. Int. J. Oncol., 2010-06-01;36(6):1469-76. 2010-06-01 [PMID: 20428771] (ICC, Human)	ICC	Human
Sniderhan LF, Garcia-Bates TM, Burgart M, Bernstein SH, Phipps RP, Maggirwar SB Neurotrophin signaling through tropomyosin receptor kinases contributes to survival and proliferation of non-Hodgkin lymphoma. Exp. Hematol., 2009-08-28;37(11):1295-309. 2009-08-28 [PMID: 19716854] (ICC, Human)	ICC	Human
Show All 15 Publications. Collapse Publications.

	Verified Customer 01/05/2015
Application:	IHC-Fr
Species:	Human

Gene Symbol	NTRK1
Uniprot	AAA36770()