I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.

Name: TrkA Antibody (315104)
Brand: Novus Biologicals
SKU: MAB1056
Price: 429 USD

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Accepted Answer

NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	RtSpecies Glossary
Applications	WB
Clone	315104
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB1056-SP

Immunogen	Mouse myeloma cell line NS0-derived recombinant rat TrkA Ala33-Pro418 Accession # P35739
Specificity	Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant human TrkA, rhTrkB, rhTrkC, rmTrkB, and rmTrkC.
Source	N/A
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	NTRK1
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

TrkA Antibody (315104)

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Publications using MAB1056	Applications	Species
V Nilaratana, DA Hauer, DE Griffin Visualization of cell-type dependent effects of anti-E2 antibody and interferon-gamma treatments on localization and expression of Broccoli aptamer-tagged alphavirus RNAs Sci Rep, 2020-03-24;10(1):5259. 2020-03-24 [PMID: 32210257] (Neutralization, Mesocricetus auratus (Hamster))	Neutralization	Mesocricetus auratus (Hamster)
V Nilaratana, DA Hauer, DE Griffin Development and characterization of Sindbis virus with encoded fluorescent RNA aptamer Spinach2 for imaging of replication and immune-mediated changes in intracellular viral RNA J. Gen. Virol., 2017-05-30;98(5):992-1003. 2017-05-30 [PMID: 28555544] (Neutralization, Hamster, Rat)	Neutralization	Hamster, Rat
Peleshok JC, Ribeiro-da-Silva A Neurotrophic factor changes in the rat thick skin following chronic constriction injury of the sciatic nerve. Mol Pain, 2012-01-10;8(0):1. 2012-01-10 [PMID: 22233577] (Western Blot, Rat)	Western Blot	Rat

Gene Symbol	NTRK1
Uniprot	P35739()