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TRAP220/MED1 Antibody (2A2)

Images

 
Western Blot: TRAP220/MED1 Antibody (2A2) [H00005469-M01] - Analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2. Lane 1: PPARBP transfected lysate (61.6 KDa). Lane ...read more
Immunocytochemistry/ Immunofluorescence: TRAP220/MED1 Antibody (2A2) [H00005469-M01] - Monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
ELISA: TRAP220/MED1 Antibody (2A2) [H00005469-M01] - Detection limit for recombinant GST tagged PPARBP is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TRAP220/MED1 Antibody (2A2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PPARBP (NP_004765, 1391 a.a. ~ 1490 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLP
Specificity
PPARBP - PPAR binding protein
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MED1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRAP220/MED1 Antibody (2A2)

  • Activator-recruited cofactor 205 kDa component
  • ARC205
  • CRSP1TRIP2
  • CRSP200p53 regulatory protein RB18A
  • DRIP230
  • DRIP230PPAR-binding protein
  • MBD4
  • MED1
  • mediator complex subunit 1DRIP205
  • mediator of RNA polymerase II transcription subunit 1
  • PBPPPARGBP
  • Peroxisome proliferator-activated receptor-binding protein
  • PPAR binding protein
  • PPARBP
  • PPARBPMGC71488
  • PPARG binding protein
  • PPARGBP
  • RB18ATR-interacting protein 2
  • Thyroid hormone receptor-associated protein complex 220 kDa component
  • thyroid hormone receptor-associated protein complex component TRAP220
  • thyroid receptor interacting protein 2
  • Thyroid receptor-interacting protein 2
  • TRAP220
  • TRAP220TRIP-2
  • TRIP2
  • vitamin D receptor-interacting protein 230 kD
  • Vitamin D receptor-interacting protein complex component DRIP205

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MED1
Entrez
OMIM
Uniprot