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Transglutaminase 2/TGM2 Antibody

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Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Transglutaminase 2/TGM2 Polyclonal Antibody (Catalog # AF5418) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Transglutaminase 2/TGM2 Polyclonal Antibody (Catalog # AF5418) followed by ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF5418
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Catalog# & Formulation Size Price
Carrier Free
AF5418-SP
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Transglutaminase 2/TGM2 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Transglutaminase 2/TGM2
Ala2-Ala686
Accession # P21981
Specificity
Detects mouse and rat Transglutaminase 2/TGM2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TGM2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5418 in the following applications:

  • ICC
    2 publications
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Transglutaminase 2/TGM2 Antibody

  • C polypeptide
  • EC 2.3.2.13
  • G-ALPHA-h
  • protein-glutamine gamma-glutamyltransferase 2
  • protein-glutamine-gamma-glutamyltransferase
  • TG(C)
  • TG2
  • TGase C
  • TGase H
  • TGase-2
  • TGase-H
  • TGC
  • TGCGNAH
  • TGM2
  • Tissue transglutaminase
  • transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 2
  • Transglutaminase C
  • Transglutaminase H
  • transglutaminase-2
  • tTG

Background

Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).

  1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  2. Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
  3. Griffin, M. et al. (2002) Biochem. J. 368:377.
  4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
  5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
  6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
  7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Transglutaminase 2/TGM2 Antibody (AF5418)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC, IHC.


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Bioinformatics

Gene Symbol TGM2
Uniprot