Reactivity | HuSpecies Glossary |
Applications | WB |
Clone | 603307 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant human TRAIL R2/TNFRSF10B Ile56-Pro128 Accession # NP_003833 |
Specificity | Detects human TRAIL R2/TNFRSF10B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhOPG, rhRANK, rhRELT, rhTNF RI, rhTNF RII, rhTRAIL R1, rhTRAIL R3, rhTRAIL R4, or rhTWEAK R is observed. Additionally in direct ELISAs, no cross‑reactivity with rhTRAIL R4, rhXEDAR, recombinant mouse (rm) DR5, rhTRAIL R1, rmTRAIL R2, or rmTNFRH3 is observed. |
Source | N/A |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Mouse |
Gene | TNFRSF10B |
Purity | Protein A or G purified from hybridoma culture supernatant |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | Protein A or G purified from hybridoma culture supernatant |
Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1 membrane protein of the TNF R family, designated TNFRSF10B, which is a receptor for TRAIL (APO2 ligand). The human TRAIL R2 cDNA encodes a 440 amino acid (aa) precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% aa sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Secondary Antibodies |
Isotype Controls |
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