Novus Biologicals products are now on bio-techne.com

TRAIL/TNFSF10 Antibody (170533)

Images

 
There are currently no images for TRAIL/TNFSF10 Antibody (MAB1121).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
170533
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

TRAIL/TNFSF10 Antibody (170533) Summary

Immunogen
E. coli-derived recombinant mouse TRAIL/TNFSF10
Pro118-Asn291
Accession # P50592
Specificity
Detects mouse TRAIL/TNFSF10 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) APRIL, rhBAFF, recombinant mouse (rm) BAFF, rhEDA-A2, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, cotton rat TNF-alpha , rhTNF-alpha , rmTNF-alpha , recombinant porcine TNF-alpha , recombinant rat TNF-alpha , recombinant rhesus macaque TNF-alpha , rhTRANCE, rhTWEAK, rhVEGI, or rhTRAIL.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TNFSF10
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Mouse TRAIL/TNFSF10 Antibody (Catalog # MAB1121)
ELISA Detection: Mouse TRAIL/TNFSF10 Biotinylated Antibody (Catalog # BAF1121)
Standard: Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL)
Publications
Read Publication using
MAB1121 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAIL/TNFSF10 Antibody (170533)

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

TNF-related apoptosis-inducing ligand (TRAIL), also called apoptosis 2 ligand (Apo2L) for its similarity in sequence, structure, and function to Fas Ligand/Apo1L, is a 33‑35 kDa type II transmembrane glycoprotein of the tumor necrosis factor superfamily, designated TNFSF10 (1‑3). Mouse TRAIL cDNA encodes a 17 amino acid (aa) N-terminal intracellular domain, a 20 aa transmembrane domain and a 253 aa extracellular domain. Like most TNF family members, TRAIL is bioactive as a homotrimer (1). Unlike other TNF family members, a zinc ion complexed by human Cys 230 (mouse Cys 240) of each of the three monomers is critical for structural stability (4, 5). Either transmembrane or cysteine protease-released soluble sTRAIL induce apoptosis of many transformed cell lines, but rarely of normal cells (3, 6). Accordingly, TRAIL is suggested to have a role in tumor surveillance (1). Mice with genetically disrupted TRAIL have defective thymocyte apoptosis, creating faulty negative selection and some increased susceptibility to induced autoimmune diseases (7). In humans, TRAIL controls apoptosis of erythrocyte precursors and sTRAIL is inversely correlated with hemoglobin (1, 8). TRAIL transcripts are constitutively expressed in a variety of human (and presumably mouse) tissues and mononuclear cells (2, 3). Only one of two receptors that transduce apoptotic signals in humans is found in the mouse (TRAIL R2/DR5 but not TRAIL R1/DR4) (1). Mice express TRAIL receptors DcTRAIL R1/TNFRSF23 and DcTRAIL R2/TNFRSF22. These receptors lack death domains, but differ in structure from human regulatory receptors TRAIL R3 and TRAIL R4 (9). Osteoprotegerin has been identified in humans as a TRAIL receptor, but binding in mouse has not yet been demonstrated (1, 10). Mouse TRAIL shows 85% aa identity with rat TRAIL and 70% aa identity with human, bovine, and porcine TRAIL within the TNF homology domain (aa 118‑291).

  1. Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
  5. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
  6. Sedger, L. M. et al. (2002) Eur. J. Immunol. 32:2246.
  7. Lamhamedi-Cherradi, S.E. et al. (2003) Nat. Immunol. 4:255.
  8. Choi, J.W. (2005) Ann. Hematol. 84:728.
  9. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  10. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56618
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
AF347
Species: Hu
Applications: IHC, Neut, WB
NB100-56116
Species: Ma, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
AF821
Species: Hu, Mu
Applications: WB
H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB120-13550
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF8221
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
126-FL/CF
Species: Hu
Applications: BA
AF860
Species: Hu, Mu
Applications: IP, Simple Western, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB100-56118
Species: Ca, Ma, Hu, Mu, Rt
Applications: Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
DY805
Species: Hu
Applications: ELISA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
MAB1121
Species: Mu
Applications: WB, ELISA(Cap)

Publications for TRAIL/TNFSF10 Antibody (MAB1121)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA Development (Capture).


Filter By Application
ELISA Development (Capture)
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for TRAIL/TNFSF10 Antibody (MAB1121) (0)

There are no reviews for TRAIL/TNFSF10 Antibody (MAB1121). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRAIL/TNFSF10 Antibody (MAB1121) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRAIL/TNFSF10 Antibody (170533) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFSF10
Uniprot