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TRA-1-60® Antibody (TRA-1-60) - Azide and BSA Free

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Western Blot: TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free [NBP2-80985] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells. Image from the standard format of this antibody.
Western Blot: TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free [NBP2-80985] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ...read more
Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free [NBP2-80985] - TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem ...read more
Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free [NBP2-80985] - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody ...read more
Western Blot: TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free [NBP2-80985] - Analysis of NB100-730 expression in NTERA-2 cl.D1 whole cell lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free [NBP2-80985] - Human embryonic stem cells stained with TRA-1-60 antibody (Cat# NB100-730) detected with Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
TRA-1-60
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-730
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TRA-1-60® Antibody (TRA-1-60) - Azide and BSA Free Summary

Immunogen
Human embryonal carcinoma cell line 2102Ep.
Localization
Cell Membrane
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA reported in scientific literature (PMID 30617132)
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

No immunoreactivity is seen with murine EC, EG or ES cells.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free

  • GCTM-2 antigen
  • GP135
  • gp200
  • PC
  • PCLP
  • PCLP-1
  • PDX
  • Podocalyxin
  • Podocalyxin-like protein 1
  • PODXL1
  • TRA160(R)
  • TRA-1-60(R)

Background

TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TRA-1-60(R)
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NBP2-80985
TRA-1-60(R) Antibody (TRA-1-60) - Azide and BSA Free
Species: Hu, Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready

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