Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, ...read more
Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using ...read more
Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # NB100-730, filled ...read more
Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Analysis of NB100-730 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Human embryonic stem cells stained with TRA-1-60 antibody (Cat# NB100-730) detected with Alexa Fluor 488 anti-mouse IgM ...read more
Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a sialidase-sensitive epitope.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
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No immunoreactivity is seen with murine EC, EG or ES cells.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified
Alternate Names for TRA-1-60(R) Antibody (TRA-1-60)
GCTM-2 antigen
GP135
gp200
PC
PCLP
PCLP-1
PDX
Podocalyxin
Podocalyxin-like protein 1
PODXL1
TRA160(R)
TRA-1-60(R)
Background
TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Lim E, Chan Y, Burns M et al. Data integration of bulk and single-cell transcriptomics from cerebral organoids and post-mortem brains to identify cell types and cell type specific driver genes in autism Research Square 2020-12-02 (FLOW, Human)
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