Novus Biologicals products are now on bio-techne.com

TRA-1-60® Antibody (TRA-1-60)

Images

 
Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, ...read more
Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using ...read more
Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # NB100-730, filled ...read more
Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Analysis of NB100-730 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Human embryonic stem cells stained with TRA-1-60 antibody (Cat# NB100-730) detected with Alexa Fluor 488 anti-mouse IgM ...read more

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
TRA-1-60
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

TRA-1-60® Antibody (TRA-1-60) Summary

Immunogen
Human embryonal carcinoma cell line 2102Ep.
Localization
Cell Membrane
Marker
Embryonic Stem Cell Marker
Specificity
Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a sialidase-sensitive epitope.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA reported in scientific literature (PMID 30617132)
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 33234532)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Control
Podocalyxin Like Overexpression Lysate
Publications
Read Publications using
NB100-730 in the following applications:

Reactivity Notes

No immunoreactivity is seen with murine EC, EG or ES cells.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60)

  • GCTM-2 antigen
  • GP135
  • gp200
  • PC
  • PCLP
  • PCLP-1
  • PDX
  • Podocalyxin
  • Podocalyxin-like protein 1
  • PODXL1
  • TRA160(R)
  • TRA-1-60(R)

Background

TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-45312
Species: Hu, Pm, Mu(-)
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00001719-M01
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
AF3159
Species: Mu
Applications: IHC
NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-64775
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, Func, IHC, IHC-Fr, IHC-P (-), IP
AF4117
Species: Rt
Applications: IHC, WB
664-LI
Species: Hu
Applications: BA
NBP3-07168
Species: Pm, Ca, Pm, Hu, Pm, Sq
Applications: Flow, ICC/IF
NBP3-35275
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
AF5866
Species: Hu
Applications: IHC, WB
NBP2-15104
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-14093
Species: Hu
Applications: IHC, IHC-P, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB100-730
Species: Hu, Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP

Publications for TRA-1-60(R) Antibody (NB100-730)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, FLOW, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(1)
FLOW
(3)
ICC/IF
(7)
IHC-P
(2)
WB
(1)
All Applications
Filter By Species
Human
(12)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-730 Applications Species
Lim E, Chan Y, Burns M et al. Data integration of bulk and single-cell transcriptomics from cerebral organoids and post-mortem brains to identify cell types and cell type specific driver genes in autism Research Square 2020-12-02 (FLOW, Human) FLOW Human
Dawes P, Smullen M, Fernandez-Fontaine A et al. FlowSeq: A multiplexed quantitative approach to identify genes affecting cell type enrichment using mosaic CRISPR-Cas9 edited cerebral organoids Research Square 2022-08-01 [PMID: 36877372]
McNeill RV, Radtke F, Nieberler M et al. Generation of four human induced pluripotent stem cells derived from ADHD patients carrying different genotypes for the risk SNP rs1397547 in the ADHD-associated gene ADGRL3 Stem cell research 2023-01-05 [PMID: 36640473] (ICC/IF, Mouse)

Details:
Dilution used in ICC 1:100
ICC/IF Mouse
Barnett DM THE CA19-9 ANTIGEN AND STRA GLYCANS DEFINE INDEPENDENT PANCREATIC DUCTAL ADENOCARCINOMA SUBPOPULATIONS IMPROVING DIAGNOSTIC ACCURACY AND APPROACH TO PROGNOSTIC CLASSIFICATION Thesis 2020-01-01
Wisniewski L, Braak S, Klamer Z et al. Heterogeneity of glycan biomarker clusters as an indicator of recurrence in pancreatic cancer Frontiers in oncology 2023-04-12 [PMID: 37124496] (IHC-P, Human) IHC-P Human
Lim ET, Chan Y, Dawes P et al. Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder Nature communications 2022-06-10 [PMID: 35688811] (FLOW, Human) FLOW Human
Nakano Y, Susa K, Yanagi T et al. Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN In vitro cellular & developmental biology. Animal 2022-02-14 [PMID: 35165826]
McDowell CT, Klamer Z, Hall J, et al. Imaging Mass Spectrometry and Lectin Analysis of N-linked Glycans in Carbohydrate Antigen Defined Pancreatic Cancer Tissues Molecular & cellular proteomics : MCP 2020-11-24 [PMID: 33234532] (IHC-P, Human) IHC-P Human
Calabrese D, Roma G, Bergling S et al. Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells PLoS One. 2019-01-01 [PMID: 31465481] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Staal B, Liu Y, Barnett D et al. The sTRA Plasma Biomarker: Blinded Validation of Improved Accuracy over CA19-9 in Pancreatic Cancer Diagnosis Clin. Cancer Res. 2019-01-07 [PMID: 30617132] (ELISA, Human) ELISA Human
Show All 17 Publications.

Reviews for TRA-1-60(R) Antibody (NB100-730) (0)

There are no reviews for TRA-1-60(R) Antibody (NB100-730). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRA-1-60(R) Antibody (NB100-730). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the host of the following antibodies, is BALC/c mouse or not?
    • The lab has informed me that these were both produced in BALB/c mice.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional TRA-1-60(R) Products

Array NB100-730

Research Areas for TRA-1-60(R) Antibody (NB100-730)

Find related products by research area.

Blogs on TRA-1-60(R)

There are no specific blogs for TRA-1-60(R), but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRA-1-60(R) Antibody (TRA-1-60) and receive a gift card or discount.