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TOR/mTOR Antibody (303728) [Unconjugated]

 (3 Publications)
 (1 Review)
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TOR was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human/Mouse TOR Monoclonal Antibody (Catalog # MAB1537) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TOR was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human/Mouse TOR Monoclonal Antibody (Catalog # MAB1537) for 3 hours at room temperature. Cells were stained with the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF
Clone
303728
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB1537-SP
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TOR/mTOR Antibody (303728) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345
Specificity
Detects human TOR in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MTOR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB1537 in the following application:
Publications
Read Publications using
MAB1537 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOR/mTOR Antibody (303728) [Unconjugated]

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

TOR, also known as FRAP1, is a member of the PI 3-kinase-related kinase (PIKK) family. It is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TOR/mTOR Antibody (MAB1537)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, Infared Microscopy, Western Blot.


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IHC
(1)
Infared Microscopy
(1)
Western Blot
(1)
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Human
(1)
Mouse
(1)
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Review for TOR/mTOR Antibody (MAB1537) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using MAB1537:
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WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot TOR/mTOR MAB1537
Enlarge 		5
reviewed by:
Verified Customer
 WB Rat 07/29/2017
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Summary

ApplicationWestern Blot
Sample Testedrat tissues
SpeciesRat

Comments

CommentsThe anti-mTOR antibody was used primary antibodies (1 : 1000; Novus Biologicals, USA).Our data showed it worked well.Antibody is good.

FAQs for TOR/mTOR Antibody (MAB1537) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Western Blot TOR/mTOR MAB1537
Enlarge
 Verified Customer
07/29/2017
Application: WB
Species: Rat
read this review

Bioinformatics

Gene Symbol MTOR
Uniprot