![Western Blot: TMEM205 Antibody [NBP1-81255] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/TMEM205-Antibody-Western-Blot-NBP1-81255-img0005.jpg "Western Blot: TMEM205 Antibody [NBP1-81255] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: RTTAAMWALQTVEKERGLGGEVPGSHQGPDPYRQLREKDPKYSALRQNFFRYH |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TMEM205 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TMEM205 |
![Immunocytochemistry/Immunofluorescence: TMEM205 Antibody [NBP1-81255] - Staining of human cell line MCF7 shows localization to nucleoplasm, nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TMEM205-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81255-img0006.jpg "Immunocytochemistry/Immunofluorescence: TMEM205 Antibody [NBP1-81255] - Staining of human cell line MCF7 shows localization to nucleoplasm, nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/TMEM205-Antibody-Immunohistochemistry-Paraffin-NBP1-81255-img0011.jpg "Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/TMEM205-Antibody-Immunohistochemistry-Paraffin-NBP1-81255-img0007.jpg "Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/TMEM205-Antibody-Immunohistochemistry-Paraffin-NBP1-81255-img0008.jpg "Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.")
![Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/TMEM205-Antibody-Immunohistochemistry-Paraffin-NBP1-81255-img0009.jpg "Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/TMEM205-Antibody-Immunohistochemistry-Paraffin-NBP1-81255-img0010.jpg "Immunohistochemistry-Paraffin: TMEM205 Antibody [NBP1-81255] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")