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TM4SF1/L6 Antibody (877621) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
877621
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

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TM4SF1/L6 Antibody (877621) [Alexa Fluor® 750] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human TM4SF1/L6
Met1-Cys202
Accession # P30408
Specificity
Detects human TM4SF1/L6 in ELISA. In Flow Cytometry, it stains HEK293 cells transfected with human TM4SF1/L6, and does not stain HEK293 cells transfected with irrelevant human cell surface marker.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TM4SF1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TM4SF1/L6 Antibody (877621) [Alexa Fluor® 750]

  • L6
  • M3s1
  • M3S1Tumor-associated antigen L6
  • Membrane component chromosome 3 surface marker 1
  • membrane component, chromosome 3, surface marker 1
  • TAAL6
  • TAAL6H-L6
  • TM4SF1
  • transmembrane 4 L six family member 1
  • transmembrane 4 L6 family member 1
  • transmembrane 4 superfamily member 1

Background

TM4SF1 (Transmembrane 4 L6 family Member 1; also L6 and M3s1) is a 23-28 kDa member of the L6 tetraspanin family of molecules. It is expressed by fibroblasts, endothelial cells and a variety of tumor cells. TM4SF1 is embedded in both the plasma membrane and the membrane of late endocytic organelles. Here, it appears to be ubiquitinated, and to regulate endocytosis, an action that impacts effective cell migration. TM4SF1 is reported to interact with alpha 5 and beta 1 integrins, and this may impact cell motility. It also suppresses the expression of CD63 and CD82, two molecules that impede cell mobility. Mouse TM4SF1 is a 202 amino acid (aa) 4‑transmembrane (TM) glycoprotein. It contains a short N-terminal cytoplasmic region (aa 1-9), followed by four TM regions and another C-terminal cytoplasmic region (aa 183-202). There is one potential isoform variant that shows an alternative start site at Met60. Over aa 116-161, mouse TM4SF1 shares 73% and 96% aa sequence identity with human and rat TM4SF1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TM4SF1
Uniprot