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TIP120A Antibody (5D7)

Images

 
Western Blot: TIP120A Antibody (5D7) [H00055832-M01] - CAND1 monoclonal antibody (M01), clone 5D7 Analysis of CAND1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TIP120A Antibody (5D7) [H00055832-M01] - Analysis of monoclonal antibody to CAND1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TIP120A Antibody (5D7) [H00055832-M01] - Analysis of monoclonal antibody to CAND1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Sandwich ELISA: TIP120A Antibody (5D7) [H00055832-M01] - Detection limit for recombinant GST tagged CAND1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
5D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TIP120A Antibody (5D7) Summary

Immunogen
CAND1 (NP_060918, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKE
Specificity
CAND1 - cullin-associated and neddylation-dissociated 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CAND1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using
H00055832-M01 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TIP120A Antibody (5D7)

  • cullin-associated and neddylation-dissociated 1
  • Cullin-associated and neddylation-dissociated protein 1
  • cullin-associated NEDD8-dissociated protein 1
  • DKFZp434M1414
  • FLJ90441
  • KIAA0829FLJ10114
  • p120 CAND1
  • TBP interacting protein
  • TBP-interacting protein 120A
  • TBP-interacting protein of 120 kDa A
  • TIP120AFLJ38691
  • TIP120FLJ10929

Background

Enhances transcription from various types of promoters . Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIP120A Antibody (H00055832-M01)(4)

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CAND1
Entrez
OMIM
Uniprot