Applications | Flow |
Clonality | Polyclonal |
Host | Sheep |
Conjugate | Alexa Fluor 488 |
Conjugate | Catalog # | Availability | Size | Price |
---|---|---|---|---|
Allophycocyanin | FAB7267A | |||
Unconjugated | AF7267 | |||
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse TIGIT Gly26-Thr143 Accession # NP_001139797 |
Specificity | Detects mouse TIGIT in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human TIGIT is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Sheep |
Gene | Tigit |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Storage | Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light. |
Buffer | Supplied in a saline solution containing BSA and Sodium Azide. |
Preservative | Sodium Azide |
TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to either PVR/CD155 or PVRL2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, TIGIT and CD226/DNAM1 pairings with PVR and PVRL2 appear to form a network that resembles the well-characterized B7-1 and B7-2 pairings with CD28 and CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity and IFN-gamma production. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is unusual in that it shows an addition of nine amino acids spread over three insertion sites. Over aa 26-143, mouse TIGIT shares only 84% aa sequence identity with rat TIGIT, but only 68% aa sequence identity with human TIGIT.
Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post. |
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Gene Symbol | Tigit |