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TIGIT Antibody (2629A) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Flow
Clone
2629A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 700

Order Details

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TIGIT Antibody (2629A) [Alexa Fluor® 700] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human embryonic kidney cell HEK293-derived Cynomolgus monkey TIGIT
Met22-Pro142
Accession # XP_00554815
Specificity
Detects Cynomolgus Monkey TIGIT in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Reconstitution Instructions
Reconstitution default not known

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIGIT Antibody (2629A) [Alexa Fluor® 700]

  • T cell immunoreceptor with Ig and ITIM domains
  • TIGIT
  • VSIG9
  • VSTM3
  • WUCAM

Background

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called VSTM3 (V-set and transmembrane domain-containing 3), VSIG9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. Cyno TIGIT shares 88.4% homology with human TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).
  1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
  2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
  3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
  4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
  5. SwissProt Accession # Q495A1.
  6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
  8. Joller, N. et al. (2011) J. Immunol. 83:1338.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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