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Thrombopoietin/THPO Antibody (34817) [Unconjugated]

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Recombinant Human Thrombo­poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega­karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo­poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo­poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND50 is typically 5‑10 µg/mL.
Recombinant Human Thrombo­poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega­karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
34817
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB288-SP
Carrier Free
MAB288-500
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Thrombopoietin/THPO Antibody (34817) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Thrombopoietin/Tpo
Ser22-Gly353
Accession # P40225
Specificity
Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
THPO
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 5-10 ug/mL
Application Notes
ELISA Detection: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog number BAF288)
ELISA Detection: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog number BAM288)
Standard: Recombinant Human Thrombopoietin/Tpo (Catalog number 288-TPN)
Publications
Read Publication using
MAB288 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombopoietin/THPO Antibody (34817) [Unconjugated]

  • Megakaryocyte colony-stimulating factor
  • Megakaryocyte growth and development factor
  • megakaryocyte stimulating factor
  • MGDF
  • MGDFC-mpl ligand
  • MKCSF
  • MK-CSF
  • ML
  • MPL ligand
  • MPLLG
  • MPLLGMGC163194
  • Myeloproliferative leukemia virus oncogene ligand
  • THCYT1
  • THPO
  • thrombopoietin nirs variant 1
  • Thrombopoietin
  • Tpo
  • TPOMKCSF

Background

Tpo is a secreted protein that regulates megakaryocytopoiesis and thrombopoiesis. It binds to and induces signaling through the Tpo receptor, which is encoded by the c-Mpl protooncogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Thrombopoietin/THPO An ...
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Thrombopoietin/THPO Antibody (34817) [Unconjugated]
Species: Hu
Applications: B/N, ELISA(Cap)

Publications for Thrombopoietin/THPO Antibody (MAB288)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Luminex Development.


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Bioinformatics

Gene Symbol THPO
Uniprot