TFCP2L1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

CP2-related proteins are a family of DNA-binding transcription factors that are generally activators of transcription and expressed ubiquitously. A novel CP2 family member, CP2-Related Transcriptional Repressor-1 (CRTR-1), is expressed in a developmentally regulated fashion in vivo and acts as a direct repressor of transcription.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-16834

• TFCP2L1 Antibodies
• TFCP2L1 Lysate
• TFCP2L1 Protein