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TdT Antibody (TDT/1393)

Images

 
Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Antibody (TDT/1393).
Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673] - Formalin-fixed, paraffin-embedded human Thymus stained with TdT Mouse Monoclonal Antibody (TDT/1393).
Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673] - Formalin-fixed, paraffin-embedded human thymoma stained with TdT Antibody (TDT/1393).
Protein Array: TdT Antibody (TDT/1393) [NBP3-07673] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TdT Monospecific Mouse Monoclonal Antibody (TDT/1393).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, MA
Clone
TDT/1393
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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TdT Antibody (TDT/1393) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08484)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment (around aa 52-192) of human TdT protein (exact sequence is proprietary) (Uniprot: P04053)
Localization
Nucleus.
Marker
Acute Lymphoblastic Leukemia Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNTT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 2-4 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TdT Antibody (TDT/1393)

  • deoxynucleotidyltransferase, terminal
  • DNTT
  • EC 2.7.7
  • EC 2.7.7.31
  • nucleosidetriphosphate:DNA deoxynucleotidylexotransferase
  • TdT
  • TDTDNA nucleotidylexotransferase
  • Terminal addition enzyme
  • Terminal deoxynucleotidyltransferase
  • terminal deoxyribonucleotidyltransferase
  • Terminal transferase

Background

TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. TdT is expressed in early lymphoid cells and by tumor cells in certain classes of acute lymphoid leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Research Areas for TdT Antibody (NBP3-07673)

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Blogs on TdT.

TdT (Terminal Deoxynucleotidyl Transferase)
The enzyme family of DNA polymerases plays a fundamental role in the replication, repair, and recombination of nucleic acid. Its members include DNA Polymerase b (Pol b), DNA Polymerase g (Pol g), and DNA Polymerase m (Pol m). TdT is a very unique and...  Read full blog post.

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Bioinformatics

Gene Symbol DNTT
Uniprot