![Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using TdT antibody (DNTT/4506R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/TdT-Antibody-DNTT-4506R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08564-img0003.jpg "Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using TdT antibody (DNTT/4506R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | DNTT/4506R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07684). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide corresponding to residues within aa1-100 of TdT was used as an immunogen. (Uniprot: P04053) |
| Localization | Nucleus. |
| Marker | Acute Lymphoblastic Leukemia Marker |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DNTT |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TdT Antibody (NBP3-08564)Find related products by research area.
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TdT (Terminal Deoxynucleotidyl Transferase) The enzyme family of DNA polymerases plays a fundamental role in the replication, repair, and recombination of nucleic acid. Its members include DNA Polymerase b (Pol b), DNA Polymerase g (Pol g), and DNA Polymerase m (Pol m). TdT is a very unique and... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DNTT |
![Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4506R).](http://images.novusbio.com/fullsize/TdT-Antibody-DNTT-4506R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08564-img0001.jpg "Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4506R).")
![Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4506R).](http://images.novusbio.com/fullsize/TdT-Antibody-DNTT-4506R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08564-img0002.jpg "Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4506R).")
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