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TdT Antibody (4B10A6) - BSA Free

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Western Blot: TdT Antibody (4B10A6) [NBP2-61899] - Analysis using DNTT mAb against HEK293 (1) and DNTT (AA: 52-192)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: TdT Antibody (4B10A6) [NBP2-61899] - Analysis of *** cells using Hela mouse mAb (green) and negative control (red).
Western Blot: TdT Antibody (4B10A6) [NBP2-61899] - Analysis using DNTT mAb against human DNTT (AA: 52-192) recombinant protein. (Expected MW is 42 kDa)
Western Blot: TdT Antibody (4B10A6) [NBP2-61899] - Analysis using DNTT mouse mAb against Raji (1), A549 (2), Hela (3), and PC-12 (4) cell lysate.
ELISA: TdT Antibody (4B10A6) [NBP2-61899] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow
Clone
4B10A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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TdT Antibody (4B10A6) - BSA Free Summary

Immunogen
Purified recombinant fragment of human TdT (AA: 52-192) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DNTT
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
Theoretical MW
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TdT Antibody (4B10A6) - BSA Free

  • deoxynucleotidyltransferase, terminal
  • DNTT
  • EC 2.7.7
  • EC 2.7.7.31
  • nucleosidetriphosphate:DNA deoxynucleotidylexotransferase
  • TdT
  • TDTDNA nucleotidylexotransferase
  • Terminal addition enzyme
  • Terminal deoxynucleotidyltransferase
  • terminal deoxyribonucleotidyltransferase
  • Terminal transferase

Background

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. ;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Research Areas for TdT Antibody (NBP2-61899)

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Blogs on TdT.

TdT (Terminal Deoxynucleotidyl Transferase)
The enzyme family of DNA polymerases plays a fundamental role in the replication, repair, and recombination of nucleic acid. Its members include DNA Polymerase b (Pol b), DNA Polymerase g (Pol g), and DNA Polymerase m (Pol m). TdT is a very unique and...  Read full blog post.

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Bioinformatics

Gene Symbol DNTT