Novus Biologicals products are now on bio-techne.com

Recombinant Human TAF148 GST (N-Term) Protein

Images

 
SDS-Page: Recombinant Human TAF148 Protein [H00009015-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, PAGE, AP

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Human TAF148 GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-450 of Human TAF1A

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSDFSEELKGPVTDDEEVETSVLSGAGMHFPWLQTYVETVAIGGKRRKDFAQTTSACLSFIQEALLKHQWQQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMESFNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILINLIQAYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPGVWDPFVKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLISVLKILYQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAKYLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKGCRYFRYILKQDHQILGKKIKRMKRSVKKYSIVNPRL

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
TAF1A
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
Theoretical MW
79.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human TAF148 GST (N-Term) Protein

  • RAFI48
  • SL1MGC:17061
  • TAFI48TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kD
  • TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
  • TATA box-binding protein-associated factor 1A
  • TATA box-binding protein-associated factor RNA polymerase I subunit A
  • TBP-associated factor 1A
  • Transcription factor SL1,48kD subunit

Background

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00006908-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP1-82545
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
H00009013-M02
Species: Hu
Applications: ELISA, S-ELISA, WB
H00009014-B01P
Species: Hu
Applications: ICC/IF, WB
NBP2-38188
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-46290
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-71822
Species: Hu
Applications: IP, WB
NB500-186
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF5866
Species: Hu
Applications: IHC, WB
NBP2-19676
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32870
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
NBP2-33466
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88582
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88376
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-15104
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB110-61646
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
H00009015-P01
Species: Hu
Applications: WB, ELISA, MA, PAGE, AP

Publications for TAF148 Recombinant Protein (H00009015-P01) (0)

There are no publications for TAF148 Recombinant Protein (H00009015-P01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TAF148 Recombinant Protein (H00009015-P01) (0)

There are no reviews for TAF148 Recombinant Protein (H00009015-P01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TAF148 Recombinant Protein (H00009015-P01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TAF148 Products

Blogs on TAF148

There are no specific blogs for TAF148, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

UBTF Antibody
NBP1-82545

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human TAF148 GST (N-Term) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol TAF1A