t-Plasminogen Activator/tPA Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (3) lacks exon 4 but the reading frame is maintained, as compared to variant 1. It encodes isoform 3 which is 46 aa shorter than isoform 1. The absence of these 46 aa makes it unlikely that isoform 3 forms a two chain disulfide linked protein like isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-14485

• t-Plasminogen Activator/tPA Antibodies
• t-Plasminogen Activator/tPA ELISA Kits
• t-Plasminogen Activator/tPA Kit
• t-Plasminogen Activator/tPA Lysates
• t-Plasminogen Activator/tPA Proteins
• t-Plasminogen Activator/tPA ELISAs
• t-Plasminogen Activator/tPA Proteins and Enzyme