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Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

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ELISA: Tissue Plasminogen Activator ELISA Kit 96Tests [NBP1-91260]
ELISA: Tissue Plasminogen Activator ELISA Kit 96Tests [NBP1-91260]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
cell culture supernatant, serum
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
6 pg/mL

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Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric) Summary

Description
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Specificity
There was no crossreactivity detected, namely not with human PAI-1 (plasminogen activator inhibitor 1, maximum concentration tested 2000 pg/ml).
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
6 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 6.5%
Intra-Assay
CV% < 3.6%
Spike Recovery
The unspiked serum was used as blank in these experiments. The recovery ranged from 77% to 109% with an overall mean recovery of 98%.
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PLAT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human t-PA
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. HRP-Conjugate anti-human t-PA polyclonal antibody
  5. Human t-PA Standard lyophilized, 2 ng/mL upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

  • Alteplase
  • DKFZp686I03148
  • EC 3.4.21
  • EC 3.4.21.68
  • plasminogen activator, tissue type
  • plasminogen activator, tissue
  • PLAT
  • Reteplase
  • tissue plasminogen activator (t-PA)
  • tissue-type plasminogen activator
  • TPA
  • T-PA
  • tPlasminogen Activator
  • t-Plasminogen Activator

Background

Tissue-type plasminogen activator (t-PA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling. Elevated levels of t-PA in serum were shown to occur in relation to retinopathy in type 1 diabetes mellitus. The major field of clinical interest is the field of diseases of the heart. t-PA plasma levels have been shown to be altered with the presence of transplant coronary artery disease in cardiac transplant recipients. A correlation between low serum levels of t-PA activity and thrombotic tendency has been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for t-Plasminogen Activator/tPA ELISA Kit (NBP1-91260). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ELISA kit that measures the active form of tPA, specifically the free unbound form in plasma. Is ELISA kit NBP1-91260 suitable?
    • Yes, this kit detects natural and recombinant human tPA and tPA complexed with PAI-1.

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  Manual - Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol PLAT