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Syntaxin 7 Antibody (CL0257)

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Orthogonal Strategies: Immunohistochemistry-Paraffin-Syntaxin 7 Antibody (CL0257)-NBP2-52893- Analysis in human lymph node and testis tissues using NBP2-52893 antibody. Corresponding STX7 RNA-seq data are ...read more
Western Blot: Syntaxin 7 Antibody (CL0257) [NBP2-52893] - Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate
Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP2-52893] - Staining of U-251 cells using the Anti-STX7 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin-Syntaxin 7 Antibody (CL0257)-NBP2-52893- Staining of human malignant melanoma shows moderate to strong cytoplasmic positivity in tumor cells.
Immunohistochemistry-Paraffin-Syntaxin 7 Antibody (CL0257)-NBP2-52893- Staining of human lymph node shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin-Syntaxin 7 Antibody (CL0257)-NBP2-52893- Staining of human kidney shows strong apical membrane positivity in cells in proximal tubules.
Immunohistochemistry-Paraffin-Syntaxin 7 Antibody (CL0257)-NBP2-52893- Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL0257
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Syntaxin 7 Antibody (CL0257) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQV
Predicted Species
Mouse (97%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STX7
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Control Peptide
Syntaxin 7 Recombinant Protein Antigen (NBP2-52893PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Syntaxin 7 Antibody (CL0257)

  • STX7
  • Syntaxin 7
  • syntaxin-7

Background

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STX7