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STAT5b Antibody (2D1)

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Western Blot: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of STAT5B expression in HeLa (Cat # L013V1).
Western Blot: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of STAT5B expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of monoclonal antibody to STAT5B on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: STAT5b Antibody (2D1) [H00006777-M03] - Analysis of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1.5 ug/ml
ELISA: STAT5b Antibody (2D1) [H00006777-M03] - Detection limit for recombinant GST tagged STAT5B is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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STAT5b Antibody (2D1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
STAT5B (NP_036580, 678 a.a. ~ 787 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS
Specificity
STAT5B (2D1)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STAT5B
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Reviewed Applications
Read 1 Review rated 1
using
H00006777-M03 in the following applications:
Publications
Read Publication using
H00006777-M03 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STAT5b Antibody (2D1)

  • signal transducer and activator of transcription 5B
  • STAT5
  • STAT5b
  • transcription factor STAT5B

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT5b Antibody (H00006777-M03)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Human
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Review for STAT5b Antibody (H00006777-M03) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00006777-M03:
Filter by Applications
Simple Western
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Simple Western STAT5b H00006777-M03
Enlarge 		1
reviewed by:
Rognvald Blance
 Simple Western Human 02/06/2017
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Summary

ApplicationSimple Western
Sample TestedK562 human chronic myelogenous leukemia cell line
SpeciesHuman
Lot11048-2D1

Comments

CommentsCells used = K562 or K562 + imatinib - lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 100 ng/µL; Gradient = 5 – 8 nested, ladder# 3; Primary antibody at 1:50; Secondary antibody = Protein Simple biotinylated anti-mouse (041-127) with Protein Simple streptavidin-HRP (041-106) both at 1:100.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STAT5b Antibody (H00006777-M03). (Showing 1 - 1 of 1 FAQ).

  1. For antibodies : STAT5b H00006777-M03; STAT 5a NBR2-00622; STAT3 NBP2-22471; STAT 6 H00006778-M01 - do these detect phosphorylated , unphosphorylated or both forms of STATs?
    • Antibodies H00006777-M03, NBR2-00622,  NBP2-22471,  and H00006778-M01 were raised to recognize total STAT proteins, they do not differentiate between any posttranslational modifications and will recognize both phosphorylated and non-phosphorylated protein. 

Secondary Antibodies

 

Isotype Controls

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Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
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Recent Reviews

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Simple Western STAT5b H00006777-M03
Enlarge
 Rognvald Blance
02/06/2017
Application: Simple Western
Species: Human
read this review

Bioinformatics

Gene Symbol STAT5B
Entrez
OMIM
Uniprot