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SNAP25 Antibody (SP12)

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Western Blot: SNAP25 Antibody (SP12) [NB600-692] - Human cell line mix using a 1:1000 dilution.
Western Blot: SNAP25 Antibody (SP12) [NB600-692] - Human cell line mix using a 1:1000 dilution.
Immunohistochemistry-Paraffin: SNAP25 Antibody (SP12) [NB600-692] - Human Cerebellar Granular Neurons stained with Mouse anti Human SNAP-25

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
SP12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-692AF350
Alexa Fluor 405
NB600-692AF405
Alexa Fluor 488
NB600-692AF488
Alexa Fluor 532
NB600-692AF532
Alexa Fluor 594
NB600-692AF594
Alexa Fluor 647
NB600-692AF647
Alexa Fluor 700
NB600-692AF700
Alexa Fluor 750
NB600-692AF750
Biotin
NB600-692B
CoraFluor 1
NB600-692CL1
DyLight 350
NB600-692UV
DyLight 405
NB600-692V
DyLight 488
NB600-692G
DyLight 550
NB600-692R
DyLight 594
NB600-692DL594
DyLight 650
NB600-692C
DyLight 680
NB600-692FR
DyLight 755
NB600-692IR
FITC
NB600-692F
HRP
NB600-692H
Janelia Fluor 525
NB600-692JF525
Janelia Fluor 549
NB600-692JF549
Janelia Fluor 646
NB600-692JF646
Janelia Fluor 669
NB600-692JF669
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SNAP25 Antibody (SP12) Summary

Immunogen
Crude human synaptic immunoprecipitate
Localization
Cytoplasmic and Plasma membrane
Specificity
SNAP25 (SP12)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNAP25
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:2000-1:5000
  • Western Blot 1:500-1:5000
Application Notes
IHC-P: Use at a dilution of 1/2000 - 1/5000. This product does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining of paraffin sections. WB: Detects a band of approximately 26-27 kDa.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivities: Rat, Porcine, Gerbil, Hamster

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for SNAP25 Antibody (SP12)

  • bA416N4.2
  • dJ1068F16.2
  • FLJ23079
  • resistance to inhibitors of cholinesterase 4 homolog
  • RIC4
  • RIC-4
  • SEC9
  • SNAP25
  • SNAP-25SNAP
  • SUP
  • Super protein
  • Synaptosomal-associated 25 kDa protein
  • synaptosomal-associated protein 25
  • synaptosomal-associated protein, 25kD
  • synaptosomal-associated protein, 25kDa

Background

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimers Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-692
SNAP25 Antibody (SP12)
Species: Hu
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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Human Brain Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

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Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.
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Bioinformatics

Gene Symbol SNAP25
Entrez
Uniprot