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SNAP25 Antibody (OTI4G5) - Azide and BSA Free

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Western Blot: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
Western Blot: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.
Flow Cytometry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SNAP25 ...read more
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI4G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74245AF350
Alexa Fluor 405
NBP2-74245AF405
Alexa Fluor 488
NBP2-74245AF488
Alexa Fluor 532
NBP2-74245AF532
Alexa Fluor 594
NBP2-74245AF594
Alexa Fluor 647
NBP2-74245AF647
Alexa Fluor 700
NBP2-74245AF700
Alexa Fluor 750
NBP2-74245AF750
Allophycocyanin
NBP2-74245APC
Allophycocyanin/Cy7
NBP2-74245APCCY7
Biotin
NBP2-74245B
CoraFluor 1
NBP2-74245CL1
DyLight 350
NBP2-74245UV
DyLight 405
NBP2-74245V
DyLight 488
NBP2-74245G
DyLight 550
NBP2-74245R
DyLight 594
NBP2-74245DL594
DyLight 650
NBP2-74245C
DyLight 680
NBP2-74245FR
DyLight 755
NBP2-74245IR
FITC
NBP2-74245F
HRP
NBP2-74245H
Janelia Fluor 525
NBP2-74245JF525
Janelia Fluor 549
NBP2-74245JF549
Janelia Fluor 646
NBP2-74245JF646
Janelia Fluor 669
NBP2-74245JF669
PE
NBP2-74245PE
PE/Atto594
NBP2-74245PEATT594
PE/Cy5.5
NBP2-74245PECY55
PE/Cy7
NBP2-74245PECY7
PerCP
NBP2-74245PCP
Unconjugated
NBP2-00795
mFluor Violet 450 SE
NBP2-74245MFV450
mFluor Violet 500 SE
NBP2-74245MFV500
mFluor Violet 610 SE
NBP2-74245MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00795
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SNAP25 Antibody (OTI4G5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SNAP25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SNAP25 Antibody (OTI4G5) - Azide and BSA Free

  • bA416N4.2
  • dJ1068F16.2
  • FLJ23079
  • resistance to inhibitors of cholinesterase 4 homolog
  • RIC4
  • RIC-4
  • SEC9
  • SNAP25
  • SNAP-25SNAP
  • SUP
  • Super protein
  • Synaptosomal-associated 25 kDa protein
  • synaptosomal-associated protein 25
  • synaptosomal-associated protein, 25kD
  • synaptosomal-associated protein, 25kDa

Background

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimers Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SNAP25 Antibody
(OTI4G5) - Azide and ...
NBP2-74245
SNAP25 Antibody (OTI4G5) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.
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Bioinformatics

Gene Symbol SNAP25