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Human, Mouse SMN ELISA Kit (Colorimetric)

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ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A typical standard curve for SMN ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of ...read more
ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A parallelism experiment was carried out to determine if the recombinant human SMN standard accurately determines SMN concentrations in biological ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell lysates
Standard Curve Range
50-3200pg/ml
Sensitivity
50pg/ml

Order Details

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Human, Mouse SMN ELISA Kit (Colorimetric) Summary

Specificity
No cross reactivity.
Standard Curve Range
50-3200pg/ml
Sensitivity
50pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
Low Concentration %CV - 11.4% Medium Concentration %CV - 8.9% High - 7.1%
Intra-Assay
Low Concentration %CV - 3.2% Medium Concentration %CV - 1.0% High - 0.8%
Spike Recovery
Human PBMC Lysate Low - 99%
Medium - 100%
High - 100%

Mouse Brain Extract Low - 104%
Medium - 86%
High - 84%

Mouse Muscle Extract Low - 125%
Medium - 88%
High - 79%

Mouse Spinal Cord Extract Low - 44%<
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SMN1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Antibody
  2. Assay Buffer 13
  3. Conjugate
  4. Extraction Reagent 4
  5. Microtiter Plate
  6. Standard
  7. Stop Solution 2
  8. TMB Substrate
  9. Wash Buffer Concentrate

Alternate Names for Human, Mouse SMN ELISA Kit (Colorimetric)

  • Gemin-1
  • Kugelberg-Welander disease)
  • SMNC
  • survival motor neuron protein
  • survival of motor neuron 1, telomeric

Background

SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for SMN ELISA Kit (NBP2-62175). (Showing 1 - 1 of 1 FAQ).

  1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
    • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

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Bioinformatics

Gene Symbol SMN1