Human, Mouse SMN ELISA Kit (Colorimetric) Summary
Specificity |
No cross reactivity. |
Standard Curve Range |
50-3200pg/ml |
Sensitivity |
50pg/ml |
Assay Type |
Sandwich ELISA |
Inter-Assay |
Low Concentration %CV - 11.4%
Medium Concentration %CV - 8.9%
High - 7.1% |
Intra-Assay |
Low Concentration %CV - 3.2%
Medium Concentration %CV - 1.0%
High - 0.8% |
Spike Recovery |
Human PBMC Lysate
Low - 99%
Medium - 100%
High - 100%
Mouse Brain Extract
Low - 104%
Medium - 86%
High - 84%
Mouse Muscle Extract
Low - 125%
Medium - 88%
High - 79%
Mouse Spinal Cord Extract
Low - 44%< |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
SMN1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Antibody
- Assay Buffer 13
- Conjugate
- Extraction Reagent 4
- Microtiter Plate
- Standard
- Stop Solution 2
- TMB Substrate
- Wash Buffer Concentrate
|
Alternate Names for Human, Mouse SMN ELISA Kit (Colorimetric)
Background
SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for SMN ELISA Kit (NBP2-62175). (Showing 1 - 1 of 1 FAQ).
-
Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
- We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.
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