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SMN Antibody (2B1) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Xp, ZeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
2B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-1936AF350
Alexa Fluor 405
NB100-1936AF405
Alexa Fluor 488
NB100-1936AF488
Alexa Fluor 532
NB100-1936AF532
Alexa Fluor 594
NB100-1936AF594
Alexa Fluor 647
NB100-1936AF647
Alexa Fluor 700
NB100-1936AF700
Alexa Fluor 750
NB100-1936AF750
Biotin
NB100-1936B
CoraFluor 1
NB100-1936CL1
DyLight 350
NB100-1936UV
DyLight 405
NB100-1936V
DyLight 488
NB100-1936G
DyLight 550
NB100-1936R
DyLight 594
NB100-1936DL594
DyLight 650
NB100-1936C
DyLight 680
NB100-1936FR
DyLight 755
NB100-1936IR
FITC
NB100-1936F
HRP
NB100-1936H
Janelia Fluor 525
NB100-1936JF525
Janelia Fluor 549
NB100-1936JF549
Janelia Fluor 585
NB100-1936JF585
Janelia Fluor 635
NB100-1936JF635
Janelia Fluor 646
NB100-1936JF646
Unconjugated
NB100-1936
mFluor Violet 450 SE
NB100-1936MFV450
mFluor Violet 500 SE
NB100-1936MFV500
mFluor Violet 610 SE
NB100-1936MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-1936
Azide and BSA Free
NBP2-80955
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SMN Antibody (2B1) [Janelia Fluor® 669] Summary

Immunogen
Purified recombinant His6-tagged human SMN protein. [Swiss-Prot# Q16637]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 27273160).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SMN Antibody (2B1) [Janelia Fluor® 669]

  • Gemin-1
  • Kugelberg-Welander disease)
  • SMNC
  • survival motor neuron protein
  • survival of motor neuron 1, telomeric

Background

SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMN Antibody (NB100-1936JF669). (Showing 1 - 1 of 1 FAQ).

  1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
    • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMN1