Home » Smad4 » Smad4 Proteins » Recombinant Human Smad4 His Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Human Smad4 His Protein

 Submit a Review

Images

 
(
Enlarge)
SDS-Page: Recombinant Human Smad4 Protein [NBC1-22616] - SMAD4, 62.6 kDa (572 aa) with a purity of 90% by SDS - PAGE
SDS-Page: Recombinant Human Smad4 Protein [NBC1-22616] - SMAD4, 62.6 kDa (572 aa) with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Novus Biologicals offers 100% Guarantee

Recombinant Human Smad4 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-552 of Human Smad4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SMAD4
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
62.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 20% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Smad4 His Protein

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4

Background

SMAD4 belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD4 promotes the binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides the function of activation required for SMAD1 or SMAD2 to stimulate transcription and may also act as a tumor suppressor. Recombinant SMAD4 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Smad2/3 Antibody
[Unconjugated]
AF3797
Western Blot Smad2/3 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) Smad2/3 Antibody [Unconjugated]
Immunocytochemistry Smad2/3 Antibody [Unconjugated]
(1 Review)
(24 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IHC, Simple Western, WB
Smad3 Antibody -
BSA Free
NB100-56479
Immunocytochemistry/ Immunofluorescence Smad3 Antibody - BSA Free
Western Blot Smad3 Antibody - BSA Free
Immunohistochemistry-Paraffin Smad3 Antibody - BSA Free
(5 Reviews)
(8 Publications)
Species: Bv, Ca, Hu, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(43 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
TGF-beta 1
[Unconjugated]
7754-BH/CF
Bioactivity TGF-beta 1 [Unconjugated]
(53 Publications)
Species: Hu
Applications: BA
Smad7 Antibody
(293039) [Unconjugate
MAB2029
Western Blot Smad7 Antibody (293039) [Unconjugated]
Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]
Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]
(1 Review)
(59 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Smad1 Antibody
[Unconjugated]
AF2039
Immunohistochemistry Smad1 Antibody [Unconjugated]
Western Blot Smad1 Antibody [Unconjugated]
Simple Western Smad1 Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
GARS Antibody
NBP3-35329
Western Blot GARS Antibody
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(191 Publications)
Species: Hu, Mu, Rt
Applications: BA
TGF-beta RI/ALK-5
Antibody [Unconjugate ...
AF3025
Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]
Immunocytochemistry TGF-beta RI/ALK-5 Antibody [Unconjugated]
Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: ELISA, ICC, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
HRAS Antibody -
BSA Free
NBP2-42864
Western Blot HRAS Antibody - BSA Free
Western Blot HRAS Antibody - BSA Free
Western Blot HRAS Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
APC Antibody - BSA
Free
NB100-91662
Immunocytochemistry/ Immunofluorescence APC Antibody - BSA Free
Flow Cytometry APC Antibody - BSA Free
Immunohistochemistry-Paraffin APC Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Smad5 [p Ser463, p
Ser465] Antibody (SY0 ...
NBP2-67394
Immunocytochemistry/ Immunofluorescence Smad5 [p Ser463, p Ser465] Antibody (SY09-03)
Immunocytochemistry/ Immunofluorescence Smad5 [p Ser463, p Ser465] Antibody (SY09-03)
Immunohistochemistry-Paraffin Smad5 [p Ser463, p Ser465] Antibody (SY09-03)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(532 Publications)
Species: Hu
Applications: BA, BA
TCEAL1 Antibody
(OTI2G10)
NBP2-46085
Immunohistochemistry TCEAL1 Antibody (OTI2G10)
Immunohistochemistry TCEAL1 Antibody (OTI2G10)
Immunohistochemistry TCEAL1 Antibody (OTI2G10)
Species: Hu
Applications: IHC, IHC-P, WB
D4S234E Antibody
(1C3) - Azide and BSA ...
H00027065-M01
Sandwich ELISA D4S234E Antibody (1C3) - Azide and BSA Free
Western Blot D4S234E Antibody (1C3) - Azide and BSA Free
Western Blot D4S234E Antibody (1C3) - Azide and BSA Free
(1 Publication)
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
Recombinant Human
Smad4 His Protein
NBC1-22616
Recombinant Human Smad4 His Protein
Species: Hu
Applications: PAGE

Publications for Smad4 Recombinant Protein (NBC1-22616) (0)

There are no publications for Smad4 Recombinant Protein (NBC1-22616).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Smad4 Recombinant Protein (NBC1-22616) (0)

There are no reviews for Smad4 Recombinant Protein (NBC1-22616). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Smad4 Recombinant Protein (NBC1-22616) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Smad4 Products

Research Areas for Smad4 Recombinant Protein (NBC1-22616)

Find related products by research area.

Blogs on Smad4

There are no specific blogs for Smad4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  NBC1-22616 MSDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Smad4 His Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol SMAD4