![Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. U251 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.](http://images.novusbio.com/fullsize/Sodium-Calcium-Exchanger-Antibody-C2C12-Immunocytochemistry-Immunofluorescence-NB120-2869-img0005.jpg "Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. U251 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Gp, I, Ma, RbSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | C2C12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Purified canine cardiac sodium/calcium exchanger. |
| Epitope | This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane. |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SLC8A3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA usage was reported in the scientific literature (PMID: 7692739). WB: Detects an approx. 120 kDa protein representing the sodium / calcium exchanger from guinea pig cardiac extract. Also detects 70 kDa and 160 kDa proteins representing a proteolytic fragment and non-reduced exchanger respectively. T-tubular membrane. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS +BSA(1mg/ml) |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
| Publications using NB120-2869 | Applications | Species |
|---|---|---|
| Yan J, Wang L, Yang Q et al. Inherited retinal degeneration: T-type voltage-gated channels, Na+/Ca2+-exchanger and calpain-2 promote photoreceptor cell death bioRxiv 2023-07-18 (IHC, Mouse) | IHC | Mouse |
| Sui S, Tian J, Gauba E et al. Cyclophilin D regulates neuronal activity-induced filopodiagenesis by fine-tuning dendritic mitochondrial calcium dynamics J. Neurochem. 2018-06-14 [PMID: 29900530] (WB) | WB | |
| Porzig H, Li Z, Nicoll DA et al. Mapping of the cardiac sodium-calcium exchanger with monoclonal antibodies. Am J Physiol;265(3 Pt 1):C748-56. 1993-09-01 [PMID: 7692739] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SLC8A3 |
![Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.](http://images.novusbio.com/fullsize/Sodium-Calcium-Exchanger-Antibody-C2C12-Immunohistochemistry-Paraffin-NB120-2869-img0006.jpg "Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.")
![Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. A2058 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.](http://images.novusbio.com/fullsize/Sodium-Calcium-Exchanger-Antibody-C2C12-Immunocytochemistry-Immunofluorescence-NB120-2869-img0003.jpg "Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. A2058 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.")
![Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. A549 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.](http://images.novusbio.com/fullsize/Sodium-Calcium-Exchanger-Antibody-C2C12-Immunocytochemistry-Immunofluorescence-NB120-2869-img0004.jpg "Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. A549 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.")
![Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.](http://images.novusbio.com/fullsize/Sodium-Calcium-Exchanger-Antibody-C2C12-Immunohistochemistry-Paraffin-NB120-2869-img0007.jpg "Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.")
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