![Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Western-Blot-NB300-578-img0015.jpg "Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).")
| Reactivity | Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, ShSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | 5F10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Purified human erythrocyte calcium ATPase. |
| Epitope | This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATP2B1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Blocking/Neutralizing and ELISA usages were reported in scientific literature. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.05% Sodium Azide |
| Purity | Unpurified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PMCA1 Antibody (NB300-578)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0006.jpg "Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0014.jpg "Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.")
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0011.jpg "Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).")
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0004.jpg "Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0005.jpg "Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0007.jpg "Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0012.jpg "Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0013.jpg "Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.")
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0010.jpg "Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).")
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