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CPS1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Analysis in human liver and kidney tissues using NBP1-86019 antibody. Corresponding CPS1 RNA-seq data are presented for the same ...read more
Western Blot: CPS1 Antibody [NBP1-86019] - Analysis in human liver tissue.
Immunocytochemistry/ Immunofluorescence: CPS1 Antibody [NBP1-86019] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Product Details

Summary
Reactivity Hu, Ca, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CPS1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPANPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSA
Predicted Species
Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CPS1 Protein (NBP1-86019PEP)
Publications
Read Publication using
NBP1-86019 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24945279).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CPS1 Antibody

  • carbamoyl-phosphate synthase [ammonia], mitochondrial
  • carbamoyl-phosphate synthase 1, mitochondrial
  • carbamoyl-phosphate synthetase 1, mitochondrial
  • carbamoylphosphate synthetase I
  • Carbamoyl-phosphate synthetase I
  • CPSase I
  • CPSASE1
  • EC 6.3.4.16

Background

The protein encoded by the CPS1 gene is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. There are two isozymes of this enzyme, and the encoded protein is the mitochondrial form. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CPS1 Antibody (NBP1-86019)(1)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Filter By Species
Canine
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on CPS1

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Bioinformatics

Gene Symbol CPS1