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Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Immunofluorescence Analysis of PFA-fixed U-87 cells using Sirtuin 2/SIRT2 Mouse Monoclonal Antibody (PCRP-SIRT2-1A8) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Immunofluorescence Analysis of PFA-fixed U-87 cells using Sirtuin 2/SIRT2 Mouse Monoclonal Antibody ...read more
Flow Cytometry: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells. Sirtuin 2/SIRT2 Mouse Monoclonal Antibody (PCRP-Sirtuin 2/SIRT2-1A8) ...read more
Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Immunofluorescence Analysis of PFA-fixed HeLa cells using Sirtuin 2/SIRT2 Mouse Monoclonal Antibody ...read more
Protein Array: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 2/SIRT2 Mouse Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, MA
Clone
PCRP-SIRT2-1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08293AF350
Alexa Fluor 405
NBP3-08293AF405
Alexa Fluor 488
NBP3-08293AF488
Alexa Fluor 532
NBP3-08293AF532
Alexa Fluor 594
NBP3-08293AF594
Alexa Fluor 647
NBP3-08293AF647
Alexa Fluor 700
NBP3-08293AF700
Alexa Fluor 750
NBP3-08293AF750
Allophycocyanin
NBP3-08293APC
Biotin
NBP3-08293B
CoraFluor 1
NBP3-08293CL1
DyLight 350
NBP3-08293UV
DyLight 405
NBP3-08293V
DyLight 488
NBP3-08293G
DyLight 550
NBP3-08293R
DyLight 594
NBP3-08293DL594
DyLight 650
NBP3-08293C
DyLight 680
NBP3-08293FR
DyLight 755
NBP3-08293IR
FITC
NBP3-08293F
HRP
NBP3-08293H
Janelia Fluor 525
NBP3-08293JF525
Janelia Fluor 549
NBP3-08293JF549
Janelia Fluor 646
NBP3-08293JF646
Janelia Fluor 669
NBP3-08293JF669
PE
NBP3-08293PE
PerCP
NBP3-08293PCP
Unconjugated
NBP3-07358-100ug
mFluor Violet 450 SE
NBP3-08293MFV450
mFluor Violet 500 SE
NBP3-08293MFV500
mFluor Violet 610 SE
NBP3-08293MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07358-100ug
Novus Biologicals offers 100% Guarantee

Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07358).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human Sirtuin 2/SIRT2 protein (Uniprot: Q8IXJ6 )
Localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIRT2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ35621
  • FLJ37491
  • S.cerevisiae, homolog) 2
  • SIR2L2
  • SIRT2
  • Sirtuin 2
  • sirtuin type 2

Background

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Sirtuin 2/SIRT2
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NBP3-08293
Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIRT2