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SIRP alpha/CD172a Antibody (602411) [Unconjugated]

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Western blot shows lysates of A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB4546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SIRP alpha /CD172a at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse ...read more
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB4546, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more
SIRPa/CD172a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
602411
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4546U-100UG
Alexa Fluor 405
FAB4546V-100UG
Alexa Fluor 488
FAB4546G-100UG
Alexa Fluor 532
FAB4546X
Alexa Fluor 594
FAB4546T-100UG
Alexa Fluor 647
FAB4546R-100UG
Alexa Fluor 700
FAB4546N-100UG
Alexa Fluor 750
FAB4546S-100UG
Allophycocyanin/Cy7
FAB4546APCCY7
Biotin
FAB4546B
CoraFluor 1
FAB4546CL1
DyLight 350
FAB4546D
DyLight 405
FAB4546E
DyLight 488
FAB4546K
DyLight 550
FAB4546L
DyLight 594
FAB4546M
DyLight 650
FAB4546W
DyLight 680
FAB4546Y
DyLight 755
FAB4546Z
HRP
FAB4546H
Janelia Fluor 525
FAB4546JF525
Janelia Fluor 549
FAB4546I
Janelia Fluor 646
FAB4546J
Janelia Fluor 669
FAB4546JF669
PE/Atto594
FAB4546PEATT594
PE/Cy5.5
FAB4546PECY55
PE/Cy7
FAB4546PECY7
Phycoerythrin
FAB4546P
mFluor Violet 450 SE
FAB4546MFV450
mFluor Violet 500 SE
FAB4546MFV500
mFluor Violet 610 SE
FAB4546MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB4546P
Carrier Free
MAB4546-SP
Carrier Free
FAB4546V-100UG
Carrier Free
FAB4546U-100UG
Carrier Free
FAB4546T-100UG
Carrier Free
FAB4546S-100UG
Carrier Free
FAB4546R-100UG
Carrier Free
FAB4546N-100UG
Carrier Free
FAB4546G-100UG
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SIRP alpha/CD172a Antibody (602411) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha /CD172a
Gly27-Asn370 (predicted)
Accession # P78324
Specificity
Detects human SIRP alpha /CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SIRPA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB4546 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP alpha/CD172a Antibody (602411) [Unconjugated]

  • BIT
  • BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
  • Brain Ig-like molecule with tyrosine-based activation motifs
  • CD172 antigen-like family member A
  • CD172a antigen
  • CD172a
  • Inhibitory receptor SHPS-1
  • Macrophage fusion receptor
  • MFR
  • MFRtyrosine phosphatase SHP substrate 1
  • MyD-1 antigen
  • MYD1
  • MYD-1
  • P84
  • protein tyrosine phosphatase, non-receptor type substrate 1
  • PTPNS1
  • SHP substrate 1
  • SHPS1
  • SHPS-1
  • SHPS1CD172A
  • signal-regulatory protein alpha
  • Signal-regulatory protein alpha-1
  • Signal-regulatory protein alpha-2
  • Signal-regulatory protein alpha-3
  • SIRP alpha
  • SIRPA
  • SIRPalpha
  • Sirp-alpha-1
  • SIRPalpha2
  • Sirp-alpha-2
  • Sirp-alpha-3
  • SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins.  It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts.  SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis.  It binds to CD47 and likely other ligands.  Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein.  It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347).  Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases.  There is one alternative start site at Met102 plus a four aa insertion after Gln421.  Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIRP alpha/CD172a Antibody (MAB4546)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol SIRPA
Uniprot