![Western Blot: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Western-Blot-NBP2-74193-img0001.jpg "Western Blot: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIL1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 52.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SIL1 |
![Immunocytochemistry/Immunofluorescence: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74193-img0004.jpg "Immunocytochemistry/Immunofluorescence: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.")
![Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Kidney tissue using anti-SIL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74193-img0007.jpg "Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Kidney tissue using anti-SIL1 mouse monoclonal antibody.")
![Flow Cytometry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - HEK293T cells transfected with either pCMV6-ENTRY SIL1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-SIL1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74193-img0002.jpg "Flow Cytometry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - HEK293T cells transfected with either pCMV6-ENTRY SIL1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-SIL1 mouse monoclonal, and then analyzed by flow cytometry.")
![Immunocytochemistry/Immunofluorescence: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of HT29 cells using anti-SIL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74193-img0003.jpg "Immunocytochemistry/Immunofluorescence: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of HT29 cells using anti-SIL1 mouse monoclonal antibody.")
![Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SIL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74193-img0005.jpg "Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SIL1 mouse monoclonal antibody.")
![Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SIL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74193-img0006.jpg "Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SIL1 mouse monoclonal antibody.")
![Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded liver tissue using anti-SIL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SIL1-Antibody-OTI3E3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74193-img0008.jpg "Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded liver tissue using anti-SIL1 mouse monoclonal antibody.")
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![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")