Home » Siglec-1/CD169 » Siglec-1/CD169 Antibodies » Siglec-1/CD169 Antibody (645608) [Phycoerythrin]
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Siglec-1/CD169 Antibody (645608) [Phycoerythrin]

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RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight were stained with Rat Anti-Mouse Siglec-1/CD169 PE-conjugated Monoclonal Antibody (Catalog # FAB5610P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight were stained with Rat Anti-Mouse Siglec-1/CD169 PE-conjugated Monoclonal Antibody (Catalog # FAB5610P, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
645608
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5610U-100UG
Alexa Fluor 405
FAB5610V-100UG
Alexa Fluor 488
FAB5610G-100UG
Alexa Fluor 532
FAB5610X
Alexa Fluor 594
FAB5610T-100UG
Alexa Fluor 647
FAB5610R-100UG
Alexa Fluor 700
FAB5610N-100UG
Alexa Fluor 750
FAB5610S-100UG
Allophycocyanin
FAB5610A
Allophycocyanin/Cy7
FAB5610APCCY7
Biotin
FAB5610B
CoraFluor 1
FAB5610CL1
DyLight 350
FAB5610D
DyLight 405
FAB5610E
DyLight 488
FAB5610K
DyLight 550
FAB5610L
DyLight 594
FAB5610M
DyLight 650
FAB5610W
DyLight 680
FAB5610Y
DyLight 755
FAB5610Z
FITC
FAB5610F
HRP
FAB5610H
Janelia Fluor 525
FAB5610JF525
Janelia Fluor 549
FAB5610I
Janelia Fluor 646
FAB5610J
Janelia Fluor 669
FAB5610JF669
PE/Atto594
FAB5610PEATT594
PE/Cy5.5
FAB5610PECY55
PE/Cy7
FAB5610PECY7
PerCP
FAB5610C
Unconjugated
MAB5610
mFluor Violet 450 SE
FAB5610MFV450
mFluor Violet 500 SE
FAB5610MFV500
mFluor Violet 610 SE
FAB5610MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5610
Carrier Free
MAB5610
Carrier Free
MAB5610-SP
Carrier Free
FAB5610V-100UG
Carrier Free
FAB5610U-100UG
Carrier Free
FAB5610T-100UG
Carrier Free
FAB5610S-100UG
Carrier Free
FAB5610R-100UG
Carrier Free
FAB5610N-100UG
Carrier Free
FAB5610G-100UG
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Siglec-1/CD169 Antibody (645608) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
Thr20-Leu1639 (predicted)
Accession # Q62230
Specificity
Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec‑1/CD169, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
SIGLEC1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB5610P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-1/CD169 Antibody (645608) [Phycoerythrin]

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Background

Siglec-1, also known as Sialoadhesin or CD169, is a 175-185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Siglec-1/CD169
Antibody (645608) [Ph ...
FAB5610P
Siglec-1/CD169 Antibody (645608) [Phycoerythrin]
Species: Mu
Applications: Flow

Publications for Siglec-1/CD169 Antibody (FAB5610P)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(2)
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Bioinformatics

Gene Symbol SIGLEC1
Uniprot