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SETD7 Antibody (OTI2D10)

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Western Blot: SETD7 Antibody (2D10) [NBP2-01066] Analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.
Western Blot: SETD7 Antibody (2D10) [NBP2-01066] Analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (2D10) [NBP2-01066] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SETD7.
Immunohistochemistry-Paraffin: SETD7 Antibody (2D10) [NBP2-01066] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody.
Flow Cytometry: SETD7 Antibody (2D10) [NBP2-01066] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SETD7 antibody, and then analyzed ...read more
Western Blot: SETD7 Antibody (2D10) [NBP2-01066] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: SETD7 Antibody (2D10) [NBP2-01066] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74145AF350
Alexa Fluor 405
NBP2-74145AF405
Alexa Fluor 488
NBP2-74145AF488
Alexa Fluor 532
NBP2-74145AF532
Alexa Fluor 594
NBP2-74145AF594
Alexa Fluor 647
NBP2-74145AF647
Alexa Fluor 700
NBP2-74145AF700
Alexa Fluor 750
NBP2-74145AF750
Allophycocyanin
NBP2-74145APC
Biotin
NBP2-74145B
CoraFluor 1
NBP2-74145CL1
DyLight 350
NBP2-74145UV
DyLight 405
NBP2-74145V
DyLight 488
NBP2-74145G
DyLight 550
NBP2-74145R
DyLight 594
NBP2-74145DL594
DyLight 650
NBP2-74145C
DyLight 680
NBP2-74145FR
DyLight 755
NBP2-74145IR
FITC
NBP2-74145F
HRP
NBP2-74145H
Janelia Fluor 525
NBP2-74145JF525
Janelia Fluor 549
NBP2-74145JF549
Janelia Fluor 646
NBP2-74145JF646
Janelia Fluor 669
NBP2-74145JF669
PE
NBP2-74145PE
PerCP
NBP2-74145PCP
mFluor Violet 450 SE
NBP2-74145MFV450
mFluor Violet 500 SE
NBP2-74145MFV500
mFluor Violet 610 SE
NBP2-74145MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74145
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SETD7 Antibody (OTI2D10) Summary

Immunogen
Full length human recombinant protein of human SETD7(NP_085151) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SETD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
40.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SETD7 Antibody (OTI2D10)

  • EC 2.1.1.364
  • EC 2.1.1.43
  • H3-K4-HMTase SETD7
  • histone H3-lysine 4-specific methyltransferase
  • histone-lysine N-methyltransferase SETD7
  • KIAA1717
  • KIAA1717SET domain-containing protein 7
  • KMT7
  • KMT7FLJ21193
  • Lysine N-methyltransferase 7
  • SET domain containing (lysine methyltransferase) 7
  • SET7
  • SET7/9
  • SET7/9Histone H3-K4 methyltransferase SETD7
  • SET7Set9
  • SET9
  • SETD7

Background

SET7 is a member of the SET (SuVar3-9, enhancer of Zeste, Trithorax) domain-containing protein family. SET7 functions as a histone methyltransferase that specifically monomethylates lysine-4 of histone H3. SET7 has also been observed to monomethylate non-histone proteins such as p53, TAF10, and the estrogen receptor. The methylating activity of SET7 is essential to cell cycle progression and genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-616
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Species: Hu
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(3E7H2) - BSA Free
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Western Blot H4/n Antibody (3E7H2) - BSA Free
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NBL1-11567
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(4 Publications)
Species: Hu, Mu
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SETD7 Antibody
(OTI2D10)
NBP2-01066
SETD7 Antibody (OTI2D10)
Species: Hu, Mu, Rt, Ca, Pm, Pm
Applications: WB, Flow, ICC/IF, IHC

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Bioinformatics

Gene Symbol SETD7
Entrez