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SETD7 Antibody (OTI2D10) - Azide and BSA Free

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Western Blot: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SETD7.
Immunohistochemistry: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody.
Flow Cytometry: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SETD7 ...read more
Western Blot: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.
Immunohistochemistry: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI2D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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SETD7 Antibody (OTI2D10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SETD7(NP_085151) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SETD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
40.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SETD7 Antibody (OTI2D10) - Azide and BSA Free

  • EC 2.1.1.364
  • EC 2.1.1.43
  • H3-K4-HMTase SETD7
  • histone H3-lysine 4-specific methyltransferase
  • histone-lysine N-methyltransferase SETD7
  • KIAA1717
  • KIAA1717SET domain-containing protein 7
  • KMT7
  • KMT7FLJ21193
  • Lysine N-methyltransferase 7
  • SET domain containing (lysine methyltransferase) 7
  • SET7
  • SET7/9
  • SET7/9Histone H3-K4 methyltransferase SETD7
  • SET7Set9
  • SET9
  • SETD7

Background

SET7 is a member of the SET (SuVar3-9, enhancer of Zeste, Trithorax) domain-containing protein family. SET7 functions as a histone methyltransferase that specifically monomethylates lysine-4 of histone H3. SET7 has also been observed to monomethylate non-histone proteins such as p53, TAF10, and the estrogen receptor. The methylating activity of SET7 is essential to cell cycle progression and genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SETD7