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Serum Amyloid A1/A2 Antibody (902728) [Unconjugated]

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Western blot shows lysates of human spleen tissue, human serum, and human plasma. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human spleen tissue, human serum, and human plasma. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by ...read more
Western blot shows 5 ng of Recombinant Human SAA1 and Recombinant Human SAA2. PVDF Membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
902728
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB30192-SP
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Serum Amyloid A1/A2 Antibody (902728) [Unconjugated] Summary

Immunogen
Human SAA1 synthetic peptide
Accession # P0DJI8
Specificity
Detects human SAA1/SAA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serum Amyloid A1/A2 Antibody (902728) [Unconjugated]

  • PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4
  • Serum Amyloid A1/A2

Background

Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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