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Serum Amyloid A1/A2 Antibody (865504) [Unconjugated]

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Recombinant human Serum Amyloid A1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30194) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30196) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant human Serum Amyloid A1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30194) coated on a Clear Polystyrene Microplate (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
865504
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
Carrier Free
MAB30196-SP
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Serum Amyloid A1/A2 Antibody (865504) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Serum Amyloid A1
Arg19-Tyr122
Accession # P0DJI8
Specificity
Detects human Serum Amyloid A1 and Serum Amyloid A2 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using
MAB30196 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serum Amyloid A1/A2 Antibody (865504) [Unconjugated]

  • PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4
  • Serum Amyloid A1/A2

Background

Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serum Amyloid A1/A2 Antibody (MAB30196)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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