![Western Blot: Senataxin Antibody [NB100-57542] - Whole cell lysate (5 ug) from HeLa, 293T, Jurkat, and A549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NB100-57542-img0006.jpg "Western Blot: Senataxin Antibody [NB100-57542] - Whole cell lysate (5 ug) from HeLa, 293T, Jurkat, and A549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP, ChIP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 875 and 925 of human senataxin using the numbering given in entry NP_055861.2 (GeneID 23064). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SETX |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in ICC/IF reported in scientific literature (PMID: 24105744). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31533039). |
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| Theoretical MW | 303 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Human | 03/22/2018 |
Summary
Comments
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reviewed by:
Patricia Richard |
WB | Human | 10/15/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Senataxin Antibody (NB100-57542)Find related products by research area.
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| Verified Customer 03/22/2018 |
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| Application: | WB | |
| Species: | Human |
| Patricia Richard 10/15/2010 |
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| Application: | WB | |
| Species: | Human |
![Immunoprecipitation: Senataxin Antibody [NB100-57542] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57542 used for IP at 3 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57543. For blotting immunoprecipitated Senataxin, NB100-57542 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunoprecipitation-NB100-57542-img0007.jpg "Immunoprecipitation: Senataxin Antibody [NB100-57542] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57542 used for IP at 3 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57543. For blotting immunoprecipitated Senataxin, NB100-57542 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Senataxin Antibody [NB100-57542] - SAN1 interacts with the RNA/DNA helicase sSenataxin. IB of SETX siRNA knockdown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05008-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Knockdown-Validated-NB100-57542-img0008.jpg "Western Blot: Senataxin Antibody [NB100-57542] - SAN1 interacts with the RNA/DNA helicase sSenataxin. IB of SETX siRNA knockdown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05008-8), licensed under a CC-BY license.")
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