![Western Blot: Semaphorin 3A Antibody [NBP3-23380] - Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Semaphorin 3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Semaphorin 3A (NBP3-23380) (lanes 1 & 2), anti-Semaphorin 3A (NBP3-23223) (lanes 3 & 4) and anti-Semaphorin 3A (NBP3-23379) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Semaphorin 3A.](http://images.novusbio.com/fullsize/nbp3-23380_rabbit-semaphorin-3a-pab-199202318202915.jpg "Western Blot: Semaphorin 3A Antibody [NBP3-23380] - Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Semaphorin 3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Semaphorin 3A (NBP3-23380) (lanes 1 & 2), anti-Semaphorin 3A (NBP3-23223) (lanes 3 & 4) and anti-Semaphorin 3A (NBP3-23379) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Semaphorin 3A.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human Semaphorin 3A. The sequence used is identical to similar regions in rat and mouse Semaphorin 3A, and has low homology to other semaphorin family members. [UniProt# Q14563] |
| Specificity | The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Semaphorin 3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera. This antibody has not been shown to be reactive in neonatal rat brain, but has weak reactivity in adult mouse brain. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SEMA3A |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Semaphorin 3A Antibody (NBP3-23380)Find related products by research area.
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| Gene Symbol | SEMA3A |
![Immunocytochemistry/Immunofluorescence: Semaphorin 3A Antibody [NBP3-23380] - Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (NBP3-23380), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23380_rabbit-semaphorin-3a-pab-199202312523614.jpg "Immunocytochemistry/Immunofluorescence: Semaphorin 3A Antibody [NBP3-23380] - Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (NBP3-23380), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
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