![Western Blot: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP2-12776-img0007.jpg "Western Blot: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 576 and 626 of human SAM Domain and HD Domain 1 using the numbering given in entry NP_056289.2 (GeneID 25939). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SAMHD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SAMHD1 Antibody (NBP2-12776)Find related products by research area.
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| Gene Symbol | SAMHD1 |
| Uniprot |
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![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-NBP2-12776-img0003.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-12776-img0005.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 (lot NBP2-12776-1) used for IP at 6 ug/mg lysate. SAMHD1 was also immunoprecipitated by another rabbit anti-SAMHD1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SAMHD1, NBP2-12776 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunoprecipitation-NBP2-12776-img0006.jpg "Immunoprecipitation: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 (lot NBP2-12776-1) used for IP at 6 ug/mg lysate. SAMHD1 was also immunoprecipitated by another rabbit anti-SAMHD1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SAMHD1, NBP2-12776 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")