Orthogonal Strategies: Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Analysis in human stomach and liver tissues. Corresponding AHCYL2 RNA-seq data are presented for the same tissues.
Western Blot: SAH3 Antibody [NBP2-47293] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NBP2-47293] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human stomach shows moderate to strong membranous positivity in parietal cells.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human small intestine shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human liver shows no positivity in hepatocytes as expected.
Mouse reactivity reported in scientific literature (PMID: 27896923).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for SAH3 Antibody
adenosylhomocysteinase-like 2
AdoHcyase 3
ADOHCYASE3
EC 3.3.1.1
FLJ21719
KIAA0828S-adenosylhomocysteine hydrolase-like 2
putative adenosylhomocysteinase 3
S-adenosylhomocysteine hydrolase-like protein 2
S-adenosyl-L-homocysteine hydrolase 3
Background
In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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