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SAH3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Analysis in human stomach and liver tissues. Corresponding AHCYL2 RNA-seq data are presented for the same tissues.
Western Blot: SAH3 Antibody [NBP2-47293] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NBP2-47293] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human stomach shows moderate to strong membranous positivity in parietal cells.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human small intestine shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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SAH3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GKVPQASAMKRSDPHHQHQRHRDGGEALVSPDGTVTEAPRTVKKQIQFADQKQEFNKRPTKI
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AHCYL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SAH3 Protein (NBP2-47293PEP)
Publications
Read Publication using
NBP2-47293 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27896923).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SAH3 Antibody

  • adenosylhomocysteinase-like 2
  • AdoHcyase 3
  • ADOHCYASE3
  • EC 3.3.1.1
  • FLJ21719
  • KIAA0828S-adenosylhomocysteine hydrolase-like 2
  • putative adenosylhomocysteinase 3
  • S-adenosylhomocysteine hydrolase-like protein 2
  • S-adenosyl-L-homocysteine hydrolase 3

Background

In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAH3 Antibody (NBP2-47293)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SAH3 Products

Array NBP2-47293

Research Areas for SAH3 Antibody (NBP2-47293)

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Blogs on SAH3

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Bioinformatics

Gene Symbol AHCYL2