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SAH3 Antibody

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Independent Antibodies: Western Blot: SAH3 Antibody [NB100-400] - Western blot and IP/WB using NB100-400. Hela whole cell lysates were probed with NB100-400 to detect endogenous SAH-3 or IPed with NB100-400 and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

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SAH3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AHCYL2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:1000-1:10000
Application Notes
This antibody may be used for Western Blot, where a band should be seen at ~70/80 kDa. It may also be used for immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-400.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAH3 Antibody

  • adenosylhomocysteinase-like 2
  • AdoHcyase 3
  • ADOHCYASE3
  • EC 3.3.1.1
  • FLJ21719
  • KIAA0828S-adenosylhomocysteine hydrolase-like 2
  • putative adenosylhomocysteinase 3
  • S-adenosylhomocysteine hydrolase-like protein 2
  • S-adenosyl-L-homocysteine hydrolase 3

Background

In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAH3 Antibody (NB100-400)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SAH3 Products

Array NB100-400

Research Areas for SAH3 Antibody (NB100-400)

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Blogs on SAH3

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Bioinformatics

Gene Symbol AHCYL2
Entrez
Uniprot